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KMID : 0350519960490020649
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Journal of Catholic Medical College
1996 Volume.49 No. 2 p.649 ~ p.659
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Chromosomal Aberrations Detected by Fluorescent In Situ Hybridization in Wilms' Tumor of Children
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Abstract
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Interphase cytogenetic analysis of paraffin-embedded tissue by fluorescent in situ hybridization (FISH) technique is a new field in solid tumor cytogenetics. We studied numerical chromosomal aberrations in 10 cases of Wilms' tumor formllin-fixed,
paraffin-embedded tissue by FISH. All 10 tissues of Wilm's tumor were obtained from male patients aged from 2 month to 7 year old. Biotin-labeled specific repetitive DNA probes for the centromeric region of chromosome 9 (D9Z1), 11 (D11Z1), 12
(D12Z3),
16 (D16Z2), and 18(D18Z1) were used. The results were s follows;
1. Aneuploidy was seen in 9 out of 10 cases (90%).
2. Among 10 cases, the most common chromosomal aberration was trisomy 9 which was found in 6 cases (60%). Trisomy 12 was found in 4 out of 10 cases (40%), monosomy 18 was found in 2 cases and monosomy 9, 11, 12, trisomy 11, or tetrasomy 12 was
found in
each 1 case respectively.
3. Seven cases (70%) showed numerical aberration in the chromosome 9 and 5 cases (50%) in the chromosome 12.
4. Diploid chromosomes were demonstrated in all cases by DNA content analysis using flow cytometry.
5. Tissue invasion was found in 5 cases (50%) and all of them revealed trisomy 9. But tissue invasion was not demonstrated in monosomy 9 (1 case) and monosomy 18 (2 cases). Numerical aberrations more than one chromosome were found in 4 cases and
3
of
them revealed tissue invasion.
We suggest that interphase cytogenetic technique using archival tissues by fluorescent in situ hybridization is the fast, simple, and reliable method for detection of numerical chromosomal aberrations in solid tumor cytogenetics and extracopy of
chromosome 9 might be one of the genetic results in tumorigenesis of the Wilins' tumor.
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